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Sino Biological™ MCAM (CD146) Recombinant Human Protein, hIgG1-Fc Tag DFS Item

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gly 559) of human MCAM precursor (NP_006491.2) fused to the Fc region of human IgG1 at the C-terminus

Manufacturer: life technologies  LS10115H02H5

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For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 10115H02H5


Description & Specifications


For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Gene ID (Entrez) 4162
Molecular Weight 86.4kDa
Purity ≥90% by SDS-PAGE
Quantity 5μg
Storage Requirements Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Structural Form Truncated/Recombinant
Protein Subtype Other Proteins
Endotoxin Concentration <1 EU/μg
Gene Alias MCAM, CD146, MUC18
Common Name Melanoma cell adhesion molecule
Gene Symbol MCAM
Protein Tag IgG1 Fc Tag
Validated Application Assay Standard
Protein Family CD Proteins
Expression System Mammalian

Melanoma cell adhesion molecule (MCAM/CD146), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in cell biology research applications. It can also be used as a protein standard.

  • Activity: Measured by the ability of the immobilized protein to support the adhesion of the HUVEC human umbilical vein endothelial cell line; when 3 x 104 cells/well are added to human CD146 coated plates (0.8μg/mL, 100μL/well), approximately >30 % will adhere after one hour at 37°C
  • Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

Shipping Condition: Room Temperature. This protein is manufactured by Sino Biological Inc.