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Sino Biological™ MCAM (CD146) Recombinant Human Protein, hIgG1-Fc Tag DFS Item


This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gly 559) of human MCAM precursor (NP_006491.2) fused to the Fc region of human IgG1 at the C-terminus

Manufacturer: life technologies  LS10115H02H25

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 10115H02H25

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Description & Specifications

Specifications

Common Name Melanoma cell adhesion molecule
Molecular Weight 86.4kDa
Protein Tag IgG1 Fc Tag
Quantity 5 x 5μg
Storage Requirements Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Validated Application Assay Standard
Protein Family CD Proteins
Protein Subtype Other Proteins
Purity ≥90% by SDS-PAGE
Endotoxin Concentration <1 EU/μg
Expression System Mammalian
Gene ID (Entrez) 4162
Gene Alias MCAM, CD146, MUC18
Structural Form Truncated/Recombinant
Gene Symbol MCAM
Format Lyophilized

Melanoma cell adhesion molecule (MCAM/CD146), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in cell biology research applications. It can also be used as a protein standard.

  • Activity: Measured by the ability of the immobilized protein to support the adhesion of the HUVEC human umbilical vein endothelial cell line; when 3 x 104 cells/well are added to human CD146 coated plates (0.8μg/mL, 100μL/well), approximately >30 % will adhere after one hour at 37°C
  • Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

Shipping Condition: Room Temperature. This protein is manufactured by Sino Biological Inc.