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Sino Biological™ LEP (Leptin) Recombinant Human Protein DFS Item

This recombinant protein was expressed from a DNA sequence encoding the mature form of human Leptin (NP_000221.1), residues 22 to 167, containing an additional Met

Manufacturer: life technologies  LS10221HNAE5

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For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 10221HNAE5


Description & Specifications


For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Gene ID (Entrez) 3952
Molecular Weight 16kDa
Purity ≥95% by SDS-PAGE
Quantity 5μg
Storage Requirements Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Structural Form Native
Protein Subtype Lectins & Leptins
Gene Alias FLJ94114, OB, OBS
Common Name Leptin
Gene Symbol LEP
Protein Tag Untagged
Validated Application Assay Standard
Protein Family Other Proteins
Expression System E. coli

Leptin (LEP), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins are suitable for use in studying protein-protein interactions, as a protein standard, or in cell biology research applications.

  • Activity: Measured by its binding ability in a functional ELISA: 1. Immobilized human Leptin at 1.25μg/mL (100μL/well) can bind human Leptin receptor-Fc with a linear range of 0.032-4.0μg/mL; 2. Immobilized human Leptin at 5μg/mL (100μL/well) can bind human Leptin receptor-His with a linear range of 0.032-4.0μg/mL
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.