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Sino Biological™ KDR (VEGFR2) Recombinant Human Protein, His Tag DFS Item


This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of human KDR (NP_002244.1) (Met 1-Glu 764) fused with a polyhistidine tag at the C-terminus

Manufacturer: life technologies  LS10012H08H50

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 10012H08H50

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Description & Specifications

Specifications

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Molecular Weight 84.6kDa
Purity ≥90% by SDS-PAGE
Quantity 50μg
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Structural Form Truncated/Recombinant/Cell Surface Protein
Protein Subtype VEGF (Vascular Endothelial Growth Factor)
Endotoxin Concentration <1 EU/μg
Gene Alias CD309, FLK1, VEGFR, VEGFR2, KDR
Common Name Kinase insert domain receptor (a type III receptor tyrosine kinase)
Gene Symbol KDR
Protein Tag His Tag
Validated Application Assay Standard
Protein Family Kinases & Inhibitors/Growth Factors & Receptors/CD Proteins
Expression System Mammalian

Kinase insert domain receptor (KDR), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.

  • Activity: Using the Octet RED System; the affinity constant (Kd) of human KDR-His bound to biotinylated human NRP2 (VEGF165) was 5.9nM
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.