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Sino Biological™ KDR (VEGFR2) Recombinant Human Protein, His Tag DFS Item


This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of human KDR (NP_002244.1) (Met 1-Glu 764) fused with a polyhistidine tag at the C-terminus

Manufacturer: life technologies  LS10012H08H50

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 10012H08H50

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Description & Specifications

Specifications

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Molecular Weight 84.6kDa
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Endotoxin Concentration <1 EU/μg
Gene Symbol KDR
Common Name Kinase insert domain receptor (a type III receptor tyrosine kinase)
Quantity 50μg
Purity ≥90% by SDS-PAGE
Gene Alias CD309, FLK1, VEGFR, VEGFR2, KDR
Protein Tag His Tag
Protein Family Kinases & Inhibitors/Growth Factors & Receptors/CD Proteins
Structural Form Truncated/Recombinant/Cell Surface Protein
Protein Subtype VEGF (Vascular Endothelial Growth Factor)
Expression System Mammalian
Validated Application Assay Standard
Format Lyophilized

Kinase insert domain receptor (KDR), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.

  • Activity: Using the Octet RED System; the affinity constant (Kd) of human KDR-His bound to biotinylated human NRP2 (VEGF165) was 5.9nM
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.