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Sino Biological™ IL1R1 Recombinant Human Protein, hIgG1-Fc Tag DFS Item


This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Thr 332) of human IL1R1 (NP_000868.1) fused to the human IgG1 Fc region at the C-terminus

Manufacturer: life technologies  LS10126H02H250

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 10126H02HL

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Description & Specifications

Specifications

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Gene ID (Entrez) 3554
Molecular Weight 63kDa
Purity ≥85% by SDS-PAGE
Quantity 5 x 50μg
Storage Requirements Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Structural Form Truncated/Recombinant
Protein Subtype Interleukins|Cytokine Receptors
Endotoxin Concentration <1 EU/μg
Gene Alias CD121A, D2S1473, IL-1R-alpha, IL1R, IL1RA, P80, IL1R1
Common Name Interleukin 1 receptor, type I
Gene Symbol IL1R1
Protein Tag IgG1 Fc Tag
Validated Application Assay Standard
Protein Family Cytokines & Receptors/CD Proteins
Expression System Mammalian

Interleukin 1 receptor, type I (IL1R1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in cell biology research applications. It is suitable for use as protein standard or in other research applications.

  • Activity: Measured by its binding ability in a functional ELISA: 1. Immobilized human IL1B at 20μg/mL (100μL/well) can bind human IL1R1-Fc with a linear range of 6.4-160ng/mL; 2. Immobilized human IL1B-His at 10μg/mL (100μL/well) can bind human IL1R1-Fc, the ED50 of human IL1R1-Fc is 0.198μg/mL; 3. Immobilized human IL1B at 10μg/mL (100μL/well) can bind human IL1R1-Fc, the ED50 of human IL1R1-Fc is 0.48μg/mL
  • Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

Shipping Condition: Room Temperature. This protein is manufactured by Sino Biological Inc.