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Sino Biological™ IL1B Recombinant Human Protein DFS Item


This recombinant protein was expressed from a DNA sequence encoding the mature form of human IL1B (NP_000567.1) (Ala 117-Ser 269)

Manufacturer: life technologies  LS10139HNAE250

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 10139HNAEL

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Description & Specifications

Specifications

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Gene ID (Entrez) 3553
Molecular Weight 17kDa
Purity ≥90% by SDS-PAGE
Quantity 5 x 50μg
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Gene Alias IL-1, IL1-BETA, IL1F2, IL1B
Common Name Interleukin 1, beta
Gene Symbol IL1B
Protein Tag Untagged
Validated Application Assay Standard
Protein Family Cytokines & Receptors
Expression System E. coli

Interleukin 1, beta (IL1B), a recombinant human protein, is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as immunogens, protein standards, and in other cell biology research applications.

  • Activity: 1.Measured by its binding ability in a functional ELISA; a. Immobilized recombinant human IL1B at 2μg/mL (100μL/well) can bind recombinant human IL1R2 with a linear range of 6.25-400ng/mL; b. Immobilized human IL1B at 10μg/mL (100μL/well) can bind human IL1R1, the EC50 of human IL1R1 is 0.48μg/mL; 2. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells, the ED50 for this effect is typically 0.5-5pg/mL
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.