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Sino Biological™ IL12A and IL12B Recombinant Mouse Protein, His Tag DFS Item


This recombinant heterodimer protein was expressed from a DNA sequence encoding the p35 subunit of human IL-12, termed as IL12A (NP_000873.2) (Met 1-Ser 219) fused to a polyhistidine tag at the C-terminus, constructed the plasmid 1, and from a DNA sequence encoding the p40 subunit of human IL-12, termed as IL12B (NP_002178.2) (Met 1-Ser 328) fused to a polyhistidine tag at the C-terminus, constructed the plasmid 2

Manufacturer: life technologies  LSCT022M08H5

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One vial of lyophilized protein

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. CT022M08H5

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Description & Specifications

Specifications

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Molecular Weight 60.3kDa
Purity ≥85% by SDS-PAGE
Quantity 5μg
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Protein Subtype Interleukins
Endotoxin Concentration <1 EU/μg
Gene Alias Interleukin-12
Common Name Interleukin 12 (interleukin 12A and interleukin 12B heterodimer)
Gene Symbol IL12A and IL12B
Protein Tag His Tag
Validated Application Assay Standard
Protein Family Cytokines & Receptors
Expression System Mammalian

Interleukin 12A and Interleukin 12B Heterodimer (IL12A and IL12B), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

  • Activity: Measured by its ability to bind biotinylated recombinant mouse IL12RB2 in a functional ELISA
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.