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Sino Biological™ IL12A and IL12B Recombinant Mouse Protein, His Tag DFS Item

This recombinant heterodimer protein was expressed from a DNA sequence encoding the p35 subunit of human IL-12, termed as IL12A (NP_000873.2) (Met 1-Ser 219) fused to a polyhistidine tag at the C-terminus, constructed the plasmid 1, and from a DNA sequence encoding the p40 subunit of human IL-12, termed as IL12B (NP_002178.2) (Met 1-Ser 328) fused to a polyhistidine tag at the C-terminus, constructed the plasmid 2

Manufacturer:  Life Technologies LSCT022M08H5

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Catalog No. CT022M08H5


 

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Includes: One vial of lyophilized protein

Specifications

Description

Protein; Sino Biological; IL12A and IL12B Recombinant Mouse Protein, His Tag; 5ug; Source: Mouse; Purity: greater than or equal to 85% by SDS-PAGE

Interleukin 12A and Interleukin 12B Heterodimer (IL12A and IL12B), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.
  • Activity: Measured by its ability to bind biotinylated recombinant mouse IL12RB2 in a functional ELISA
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.

Description & Specifications

Specifications

Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
60.3kDa
5μg
Interleukins
Interleukin-12
IL12A and IL12B
Cytokines & Receptors
His Tag
Lyophilized
≥85% by SDS-PAGE
Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
<1 EU/μg
Interleukin 12 (interleukin 12A and interleukin 12B heterodimer)
Assay Standard
Mammalian