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Sino Biological™ IL12A and IL12B Recombinant Human Protein, His Tag DFS Item


This recombinant heterodimer protein was expressed from a DNA sequence encoding the p35 subunit of human IL-12, termed as IL12A (NP_000873.2) (Met 1-Ser 219) fused to a polyhistidine tag at the C-terminus, constructed the plasmid 1, and from a DNA sequence encoding the p40 subunit of human IL-12, termed as IL12B (NP_002178.2) (Met 1-Ser 328) fused to a polyhistidine tag at the C-terminus, constructed the plasmid 2

Manufacturer: life technologies  LSCT011H08H5

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One vial of lyophilized protein

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. CT011H08H5

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Description & Specifications

Specifications

Quantity 5μg
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Gene Symbol IL12A and IL12B
Validated Application Assay Standard
Molecular Weight 60.2kDa
Protein Tag His Tag
Expression System Mammalian
Protein Subtype Interleukins
Common Name Interleukin 12 (interleukin 12A and interleukin 12B heterodimer)
Gene Alias Interleukin-12
Protein Family Cytokines & Receptors
Endotoxin Concentration <1 EU/μg
Purity ≥90% by SDS-PAGE
Format Lyophilized

Interleukin 12 (Interleukin 12A and Interleukin 12B Heterodimer) (IL12A and IL12B), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

  • Activity: Measured by its ability to bind biotinylated recombinant human IL12RB1 in a functional ELISA
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.