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Sino Biological™ IGFBP3 Recombinant Human Protein, His Tag DFS Item

This recombinant protein was expressed from a DNA sequence encoding the mature form of human IGFBP3 (NP_000589.2), residues 28 to 291, fused to a polyhistidine tag at the N-terminus

Manufacturer: life technologies  LS10430H07H50

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For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 10430H07H50


Description & Specifications


For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Gene ID (Entrez) 3486
Molecular Weight 31kDa
Purity ≥85% by SDS-PAGE
Quantity 50μg
Storage Requirements Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Structural Form Recombinant
Protein Subtype IGFs (Insulin-Like Growth Factors)
Endotoxin Concentration <1 EU/μg
Gene Alias IGFBP3, tcag7.703, BP-53, IBP3
Common Name Insulin-like growth factor binding protein 3
Gene Symbol IGFBP3
Protein Tag His Tag
Validated Application Assay Standard
Protein Family Growth Factors & Receptors
Expression System Mammalian

Insulin-like growth factor binding protein 3 (IGFBP3), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins are suitable for use in studying protein-protein interactions, as a protein standard, or in cell biology research applications.

  • Activity: Measured by its binding ability in a functional ELISA: 1. Immobilized human IGF2 at 10μg/mL (100μL/well) can biotinylated human IGFBP3-His, the ED50 of can biotinylated human IGFBP3-His is 18ng/mL; 2. Immobilized human IGF1 at 10μg/mL (100μL/well) can biotinylated human IGFBP3-His, the ED50 of can biotinylated human IGFBP3-His is 24ng/mL
  • Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

Shipping Condition: Room Temperature. This protein is manufactured by Sino Biological Inc.