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Sino Biological™ IGF2 Recombinant Human Protein, His.NusA Tag DFS Item


This recombinant protein was expressed from a DNA sequence encoding the mature form of human IGF2 (P01344-1) (Ala 25-Glu 91) fused to the polyhistidine-NusA tag at the N-terminus

Manufacturer: life technologies  LS13032H24E5

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 13032H24E5

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Description & Specifications

Specifications

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Molecular Weight 64.6kDa
Purity ≥85% by SDS-PAGE
Quantity 5μg
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Structural Form Full Length/Recombinant
Protein Subtype IGFs (Insulin-Like Growth Factors)
Gene Alias PP1446, C11orf43, FLJ22066, FLJ44734, IGF-II, PP9974
Common Name Insulin-like growth factor 2 (somatomedin A)
Gene Symbol IGF2
Protein Tag His Tag/NusA Tag
Validated Application Assay Standard
Protein Family Growth Factors & Receptors
Expression System E. coli

Insulin-like growth factor 2 (IGF2), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins are suitable for use in studying protein-protein interactions, as a protein standard, or in cell biology research applications.

  • Activity: Measured by its binding ability in a functional ELISA; immobilized human IGF2 at 10μg/mL (100μL/well) can bind biotinylated human His-IGFBP3; the EC50 of binding to biotinylated human His-IGFBP3 is 18ng/mL
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.