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Sino Biological™ ICAM1 Recombinant Human Protein, His Tag DFS Item


This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of human ICAM1 (NP_000192.2) (Met 1 - Glu 480) fused to a polyhistidine tag at the C-terminus

Manufacturer: life technologies  LS10346H08H25

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 10346H08H25

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Description & Specifications

Specifications

Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Molecular Weight 51kDa
Protein Subtype Intercellular Adhesion Molecule
Gene Alias BB2, CD54, P3.58
For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Common Name Intercellular adhesion molecule 1
Format Lyophilized
Endotoxin Concentration <1 EU/μg
Gene Symbol ICAM1
Validated Application Assay Standard
Structural Form Truncated/Recombinant/Cell Surface Protein
Quantity 5 x 5μg
Purity ≥90% by SDS-PAGE
Protein Tag His Tag
Protein Family CD Proteins
Expression System Mammalian

Intercellular adhesion molecule 1 (ICAM1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.

  • Activity: Measured by the ability of the immobilized protein to support the adhesion of PMA stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells; when cells are added to ICAM1 coated plates (12.5μg/mL, 100μL/well), approximately 20-40% will adhere specifically
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.