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Sino Biological™ HTRA2 Recombinant Human Protein, His Tag DFS Item


This recombinant protein was expressed from a DNA sequence encoding the mature form of human HTRA2 (O43464-1) (Ala 134-Glu 458) fused to a polyhistide tag at the C-terminus

Manufacturer: life technologies  LS10619H08E25

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 10619H08E25

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Description & Specifications

Specifications

Quantity 5 x 5μg
Purity ≥85% by SDS-PAGE
For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Gene Symbol HTRA2
Storage Requirements Store at -80°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Protein Tag His Tag
Expression System E. coli
Validated Application Assay Standard
Gene Alias OMI, PARK13, PRSS25
Protein Subtype Proteases
Format Frozen
Common Name HtrA serine peptidase 2
Molecular Weight 36.5kDa

HTRA Serine Peptidase 2 (HTRA2), recombinant human protein is supplied in frozen format. It is suitable for use in protein studies such as enzymatic analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

  • Activity: Protease activity demonstrated by HTRA2 cleavage of bovine beta-casein (Sigma Cat. No. C-6905); incubation of beta-casein at 0.2μg/mL with Recombinant Human HTRA2 at 0.02μg/mL (ratio of 10:1) for 60 minutes at 45°C in 50mM Tris, pH 8.0, which results in >95% cleavage of beta-casein, as revealed by SDS-PAGE
  • Formulation: 50mM Tris, 0.3M NaCl, 1mM DTT, 0.2μg/μL BSA, 20% glycerol, pH 7.8
  • Reconstitution: No reconstitution needed

This protein is manufactured by Sino Biological Inc.