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Sino Biological™ HMGB1 Recombinant Human Protein, hIgG1-Fc Tag DFS Item

This recombinant protein is expressed from a DNA sequence encoding the human HMGB1 protein (NP_002119.1) (Met 1-Glu 215) fused to the fused Fc region of human IgG1 at the N-terminus

$150.15 - $718.20

Specifications

Purity ≥90% by SDS-PAGE
Endotoxin Concentration <1 EU/μg
Format Lyophilized
Gene Alias DKFZp686A04236, HMG1, HMG3, SBP-1, HMGB1
View More Specs

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

10326H01H25

DFS Item
 
life technologies
LS10326H01H25
5 x 5μg Each for $397.95

10326H01H5

DFS Item
 
life technologies
LS10326H01H5
5μg Each for $150.15

10326H01H50

DFS Item
 
life technologies
LS10326H01H50
50μg Each for $718.20
Description & Specifications

Specifications

Purity ≥90% by SDS-PAGE
Endotoxin Concentration <1 EU/μg
Format Lyophilized
Gene Alias DKFZp686A04236, HMG1, HMG3, SBP-1, HMGB1

High Mobility Group Box 1 (HMGB1) recombinant human protein is supplied as a lyophilized powder. This protein is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.&line-break;Background: Human High-Mobility Group Box 1 protein (HMGB1), also known as HMG-1 or amphoterin previously, is a member of the HMGB family consisting of three members, HMGB1, HMGB2 and HMGB3. HMGB1 is a nonhistone architectural chromosomal protein ubiquitously present in all vertebrate nuclei and binds double-stranded DNA without sequence specificity. HMGB1 is structurally composed of two homologous DNA-binding domains entitled box A and box B and a highly negative-charged C terminus. This protein is extremely conserved across species with 99- 100% aa sequence homology to mouse, rat, bovine, canine, and porcine proteins. As a nuclear protein, HMGB1 stabilizes nucleosomes and allows bending of DNA that facilitates gene transcription which is essential for individual survival. Meanwhile, it is revealed that HMGB1 can also act as a cytokine extracellularly and regulates monocyte, T cell, dendritic cell activities in inflammatory responses. Once secreted, HMGB1 induces signal transduction through its receptor RAGE and perhaps TLR2 and TLR4. The immune-regulatory function of HMGB1 may lead to being identified as new therapeutic target.

  • N-terminal Sequence Analysis: Glu 20
  • Activity: measured by its binding ability in a functional ELISA; immobilized recombinant mouse AGER at 2μg/mL (100μL/well) has been shown to bind human HMGB1, the EC50 of human HMGB1 is 0.23μg/mL
  • Formulation: Reconstitution: dissolve the protein in sterile double-distilled water to a concentration of 25mg/mL or lower; aliquot and use as soon as possible

Manufactured by Sino Biological (China).