Sino Biological™ HMGB1 Recombinant Human Protein, hIgG1-Fc Tag
This recombinant protein is expressed from a DNA sequence encoding the human HMGB1 protein (NP_002119.1) (Met 1-Glu 215) fused to the fused Fc region of human IgG1 at the N-terminus
Manufacturer: Life Technologies LS10326H01H5
Protein; Sino Biological; HMGB1 Recombinant Human Protein, hIgG1-Fc Tag; 5ug; Source: Human; Purity: greater than or equal to 90% by SDS-PAGEHigh Mobility Group Box 1 (HMGB1) recombinant human protein is supplied as a lyophilized powder. This protein is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.&line-break;Background: Human High-Mobility Group Box 1 protein (HMGB1), also known as HMG-1 or amphoterin previously, is a member of the HMGB family consisting of three members, HMGB1, HMGB2 and HMGB3. HMGB1 is a nonhistone architectural chromosomal protein ubiquitously present in all vertebrate nuclei and binds double-stranded DNA without sequence specificity. HMGB1 is structurally composed of two homologous DNA-binding domains entitled box A and box B and a highly negative-charged C terminus. This protein is extremely conserved across species with 99- 100% aa sequence homology to mouse, rat, bovine, canine, and porcine proteins. As a nuclear protein, HMGB1 stabilizes nucleosomes and allows bending of DNA that facilitates gene transcription which is essential for individual survival. Meanwhile, it is revealed that HMGB1 can also act as a cytokine extracellularly and regulates monocyte, T cell, dendritic cell activities in inflammatory responses. Once secreted, HMGB1 induces signal transduction through its receptor RAGE and perhaps TLR2 and TLR4. The immune-regulatory function of HMGB1 may lead to being identified as new therapeutic target.
- N-terminal Sequence Analysis: Glu 20
- Activity: measured by its binding ability in a functional ELISA; immobilized recombinant mouse AGER at 2μg/mL (100μL/well) has been shown to bind human HMGB1, the EC50 of human HMGB1 is 0.23μg/mL
- Formulation: Reconstitution: dissolve the protein in sterile double-distilled water to a concentration of 25mg/mL or lower; aliquot and use as soon as possible
Manufactured by Sino Biological (China).
|Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories|
|DKFZp686A04236, HMG1, HMG3, SBP-1, HMGB1|
|Cytokines & Receptors|
|IgG1 Fc Tag|
|≥90% by SDS-PAGE|
|Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.|
|High mobility group box 1|