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Sino Biological™ GFP Recombinant Aequorea victoria Protein, N-His Tag DFS Item


This recombinant protein is expressed from a DNA sequence encoding the Aequorea victoria GFP (AAB65663) (Ser 2-Lys 238) fused to a polyhistidine tag at the N-terminus

Manufacturer: life technologies  LS13105S07E50

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 13105S07E50

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Description & Specifications

Specifications

Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Termination N-Terminal
Expression System E. coli
Molecular Weight 28.7kDa
Common Name Green Fluorescent Protein
Gene Symbol GFP
Gene Alias GFP
Format Lyophilized
For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Purification Method PAGE
Protein Family Other Proteins
Validated Application Assay Standard
Structural Form Full Length/Recombinant
Quantity 50μg
Purity ≥85% by SDS-PAGE
Protein Tag His Tag

Green Fluorescent Protein (GFP) recombinant Aequorea victoria protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein structure analysis and in cell biology research applications.&line-break;Background: The green fluorescent protein (GFP) is a protein that exhibits bright green fluorescence when exposed to blue light. The protein is in the shape of a cylinder, comprising 11 strands of β-sheet with an α-helix inside and short helical segments on the ends of the cylinder. Its ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. The green-fluorescent protein (GFP) of the jellyfish Aequorea victoria has long been used as a universal reporter in a broad range of heterologous living cells and organisms. GFP has become well established as a marker of gene expression and protein targeting in intact cells and organisms.

  • N-terminal Sequence Analysis: Glu 99
  • Activity: 1. Mesured by emission analysis; 2. Measure excitation and emission spectra of GFP at wavelengths of 395nM and 510nM respectively
  • Formulation: Lyophilized from sterile PBS, pH 7.5
  • Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

Manufactured by Sino Biological (China).