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Sino Biological™ GFP Recombinant Aequorea victoria Protein, N-His Tag DFS Item


This recombinant protein is expressed from a DNA sequence encoding the Aequorea victoria GFP (AAB65663) (Ser 2-Lys 238) fused to a polyhistidine tag at the N-terminus

Manufacturer: life technologies  LS13105S07E25

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 13105S07E25

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Description & Specifications

Specifications

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Molecular Weight 28.7kDa
Purification Method PAGE
Purity ≥85% by SDS-PAGE
Quantity 5 x 5μg
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Termination N-Terminal
Structural Form Full Length/Recombinant
Gene Alias GFP
Common Name Green Fluorescent Protein
Gene Symbol GFP
Protein Tag His Tag
Validated Application Assay Standard
Protein Family Other Proteins
Expression System E. coli

Green Fluorescent Protein (GFP) recombinant Aequorea victoria protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein structure analysis and in cell biology research applications.&line-break;Background: The green fluorescent protein (GFP) is a protein that exhibits bright green fluorescence when exposed to blue light. The protein is in the shape of a cylinder, comprising 11 strands of β-sheet with an α-helix inside and short helical segments on the ends of the cylinder. Its ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. The green-fluorescent protein (GFP) of the jellyfish Aequorea victoria has long been used as a universal reporter in a broad range of heterologous living cells and organisms. GFP has become well established as a marker of gene expression and protein targeting in intact cells and organisms.

  • N-terminal Sequence Analysis: Glu 99
  • Activity: 1. Mesured by emission analysis; 2. Measure excitation and emission spectra of GFP at wavelengths of 395nM and 510nM respectively
  • Formulation: Lyophilized from sterile PBS, pH 7.5
  • Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

Manufactured by Sino Biological (China).