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Sino Biological™ FCGR3A (CD16a), 176 Phe/Val Recombinant Human Protein, His.AVI Tag, Biotinylated DFS Item


This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gln 208) of human FCGR3A (P08637-1), containing the 176 Phe/Val mutation, fused to a polyhistidine and AVI tags at the C-terminus

Manufacturer: life technologies  LS10389H27H1B25

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 10389H27H1M

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Description & Specifications

Specifications

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Gene ID (Entrez) 2214
Molecular Weight 25.6kDa
Purity ≥90% by SDS-PAGE
Quantity 5 x 5μg
Storage Requirements Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Structural Form Truncated/Recombinant/Cell Surface Protein
Protein Subtype Glycoproteins|Fc Receptors
Endotoxin Concentration <1 EU/μg
Gene Alias RP11-5K23.1, CD16, CD16A, FCG3, FCGR3, FCGRIII, FCR-10, FCRIII, FCRIIIA, IGFR3
Common Name Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Gene Symbol FCGR3A
Protein Tag His Tag/AVI Tag
Validated Application Assay Standard
Protein Family Growth Factors & Receptors/CD Proteins
Expression System Mammalian

Fc gamma receptor III-a (FCGR3A/CD16a), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in studying protein-protein interactions, and cell biology research applications. It can also be used as a protein standard. This protein is biotinylated.

  • Activity: Measured by its binding ability in a functional ELISA; immobilized Protein A at 0.5μg/well can bind human IgG1 with a linear range of 0.078-0.625μg/mL with 3mg/mL of biotinylated human FCGR3A
  • Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

Shipping Condition: Room Temperature. This protein is manufactured by Sino Biological Inc.