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Sino Biological™ FCGR2A (CD32A) Recombinant Human Protein, His.AVI Tag, Biotinylated DFS Item

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Met 210) of human FCGR2A (AAA35827.1) (167Arg/His) fused to a C-terminal polyhistidine tagged AVI tag at the C-terminus

Manufacturer: life technologies  LS10374H27H1B25

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For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 10374H27H1L


Description & Specifications


For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Molecular Weight 23.6kDa
Purity ≥90% by SDS-PAGE
Quantity 5 x 5μg
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Protein Subtype Fc Receptors
Endotoxin Concentration <1 EU/μg
Gene Alias RP11-5K23.6, CD32, CD32A, CDw32, FCG2, FCGR2, FCGR2A, FCGR2A1, FcGR, IGFR2, mgC23887
Common Name Fc gamma receptor II-a
Gene Symbol FCGR2A
Protein Tag His Tag/AVI Tag
Validated Application Assay Standard
Protein Family CD Proteins
Expression System Mammalian

Fc Gamma Receptor II-A (FCGR2A), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins are suitable for use in studying protein-protein interactions, as a protein standard, or in cell biology research applications.

  • The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme)
  • Activity: 1.Measured by its binding ability in a functional ELISA; immobilized human IgG2 at 10μg/mL (100μL/well) can bind biotinylated human FCGR2A with a linear range of 0.16-0.8μg/mL
  • 2. Using the Octet RED System, the recombinant human FCGR2A and BirA bound to human IgG; the affinity constant (Kd) was 12nM
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.