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Sino Biological™ FCGR1A (CD64) Recombinant Human Protein, His Tag DFS Item


This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Pro 288) of human FCGR1A (NP_000557.1) fused to a polyhistidine tag at the C-terminus

Manufacturer: life technologies  LS10256H08H5

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 10256H08H5

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Description & Specifications

Specifications

Validated Application Assay Standard
For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Purity >85% by SDS-PAGE
Common Name Fc fragment of IgG, high affinity Ia, receptor (CD64)
Endotoxin Concentration <1 EU/μg
Expression System Mammalian
Protein Tag His Tag
Protein Family Growth Factors & Receptors/CD Proteins
Protein Subtype Glycoproteins|Fc Receptors
Quantity 5μg
Structural Form Truncated/Recombinant/Cell Surface Protein
Gene Alias FCGR1A, CD64, CD64A, FCRI, FLJ18345, IGFR1
Format Lyophilized
Gene ID (Entrez) 2209
Molecular Weight 32kDa
Storage Requirements Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Gene Symbol FCGR1A

Fc gamma receptor I-a (FCGR1A/CD64), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.

  • Activity: Measured by its binding ability in a functional ELISA: 1. Immobilized CD64 at 10μg/mL can bind human IgG1 with a linear range of 0.39-25ng/mL; 2. Scatchard analysis showed that the affinity constant (Kd) of CD64 bound to human IgG1 was 0.059nM
  • Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.