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Sino Biological™ FCGR1A (CD64) Recombinant Human Protein, His Tag DFS Item


This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Pro 288) of human FCGR1A (NP_000557.1) fused to a polyhistidine tag at the C-terminus

Manufacturer: life technologies  LS10256H08H250

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 10256H08HL

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Description & Specifications

Specifications

Common Name Fc fragment of IgG, high affinity Ia, receptor (CD64)
Gene Alias FCGR1A, CD64, CD64A, FCRI, FLJ18345, IGFR1
Validated Application Assay Standard
Format Lyophilized
Gene ID (Entrez) 2209
Protein Tag His Tag
Purity >85% by SDS-PAGE
Quantity 5 x 50μg
For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Molecular Weight 32kDa
Expression System Mammalian
Structural Form Truncated/Recombinant/Cell Surface Protein
Gene Symbol FCGR1A
Endotoxin Concentration <1 EU/μg
Protein Subtype Glycoproteins|Fc Receptors
Protein Family Growth Factors & Receptors/CD Proteins
Storage Requirements Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.

Fc gamma receptor I-a (FCGR1A/CD64), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.

  • Activity: Measured by its binding ability in a functional ELISA: 1. Immobilized CD64 at 10μg/mL can bind human IgG1 with a linear range of 0.39-25ng/mL; 2. Scatchard analysis showed that the affinity constant (Kd) of CD64 bound to human IgG1 was 0.059nM
  • Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.