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Sino Biological™ EGFR Recombinant Human Protein (without Catalytic Activity Domain), hIgG1-Fc Tag DFS Item


This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gly 645) of human EGFR (NP_005219) fused to the Fc region of human IgG1 at the C-terminus

Manufacturer: life technologies  LS10001H02H50

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 10001H02H50

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Description & Specifications

Specifications

Protein Subtype Receptor Tyrosine Kinases|Growth Factor Receptors
Gene Alias EGF R, ErbB, HER1, mENA, ErbB1, PIG61
Common Name Epidermal growth factor receptor
Protein Family Growth Factors & Receptors
Expression System Mammalian
For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Gene ID (Entrez) 1956
Molecular Weight 95kDa
Purity ≥85% by SDS-PAGE
Quantity 50μg
Storage Requirements Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Structural Form Truncated/Recombinant
Endotoxin Concentration <1 EU/μg
Gene Symbol EGFR
Protein Tag IgG1 Fc Tag
Validated Application Assay Standard
KinaseFamily EGF Receptor (ErbB) Kinase Family

Epidermal growth factor receptor (EGFR), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in studying protein-protein interactions, and cell biology research applications. It can also be used as a protein standard. Since this product is a truncated protein and does not contain intracellular catalytic activity domain, it is not suitable for use in enzyme activity studies.

  • Activity: Measured by its binding ability in a functional ELISA; immobilized recombinant human EGF at 10μg/mL (100μL/well) can bind human EGFR with a linear range of 0.64-400ng/mL
  • Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween-80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.