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Sino Biological™ DKK1 Recombinant Human Protein, His Tag DFS Item


This recombinant protein was expressed from a DNA sequence encoding the human DKK1 precursor (NP_036374.1) (Met 2-His 266) fused to a polyhistidine tag at the C-terminus

Manufacturer: life technologies  LS10170H08H5

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 10170H08H5

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Description & Specifications

Specifications

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Molecular Weight 25.8kDa
Purity ≥85% by SDS-PAGE
Quantity 5μg
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Protein Subtype Cytokine Receptors
Endotoxin Concentration <1 EU/μg
Gene Alias SK, DKK-1
Common Name Dickkopf 1 homolog (Xenopus laevis)
Gene Symbol DKK1
Protein Tag His Tag
Validated Application Assay Standard
Protein Family Other Proteins
Expression System Mammalian

Dickkopf 1 homolog (Xenopus laevis) (DKK1), recombinant rhesus protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.

  • Activity: Measured by its ability to inhibit WNT3A-induced alkaline phosphatase production by C3H10T1/2 cells; the ED50 for this effect is approximately 0.1-0.4μg/mL in the presence of 100ng/mL of mouse Wnt3a
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.