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Sino Biological™ CTLA4 Recombinant Human Protein, His Tag DFS Item


This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of human CTLA4 (NP_005205.2) (Met 1-Phe 162) fused to a polyhistidine tag at the C-terminus

Manufacturer: life technologies  LS11159H08H250

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 11159H08HL

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Description & Specifications

Specifications

Quantity 5 x 50μg
Purity ≥90% by SDS-PAGE
Common Name Cytotoxic T-lymphocyte-associated protein 4
Validated Application Assay Standard
Expression System Mammalian
Endotoxin Concentration <1 EU/μg
Gene Alias CTLA4, CD, CD152, CELIAC3, CTLA-4, GRD4, GSE, ICOS, IDDM12
Molecular Weight 15kDa/25kDa (post-glycosylation)
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Protein Subtype Other Proteins
Gene Symbol CTLA4
Protein Tag His Tag
Protein Family CD Proteins
Format Lyophilized
For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories

Cytotoxic T-lymphocyte-associated protein 4 (CTLA4), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

  • Activity: Measured by its binding ability in a functional ELISA: 1. Immobilized human CTLA4 at 10μg/mL (100μL/well) can bind human CD80-Fc, the EC50 of human CD80-Fc is 14ng/mL; 2. Immobilized human CTLA4 at 10μg/mL (100μL/well) can bind human CD86-His-Fc, the EC50 of human CD86-His-Fc is 53ng/mL
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.