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Sino Biological™ CPA2 Recombinant Human Protein, His Tag DFS Item


This recombinant protein was expressed from a DNA sequence encoding the pre pro form of human CPA2 (NP_001859.1) (Met 1-Tyr 417) fused to a polyhistidine tag at the C-terminus

Manufacturer: life technologies  LS10499H08H50

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 10499H08H50

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Description & Specifications

Specifications

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Molecular Weight 46kDa
Purity ≥85% by SDS-PAGE
Quantity 50μg
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Protein Subtype Proteases
Endotoxin Concentration <1 EU/μg
Gene Alias CPA2
Common Name Carboxypeptidase A2 ( CPA2 ) Protein
Gene Symbol CPA2
Protein Tag His Tag
Validated Application Assay Standard
Protein Family Other Proteins
Expression System Mammalian

Carboxypeptidase A2 (pancreatic) (CPA2), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

  • Activity: Measured by its ability to cleave a colorimetric peptide substrate, N-acetyl-Phe-Thiaphe-OH (N-Ac-PSP, Peptide International Cat. No. STP-3621-PI), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB), as measured using the wavelength at 405nM and the extinction coefficient of 13,260 M-1 cm-1, the specific activity is >4nM/min./μg
  • Formulation: Lyophilized in 25mM Tris, 0.15M NaCl, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.