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Sino Biological™ CNTF Recombinant Human Protein, N-His Tag DFS Item


This recombinant protein was expressed from a DNA sequence encoding the human CNTF (NP_000605.1) (Ala 2-Met 200) fused to a polyhistide tag at the N-terminus

Manufacturer: life technologies  LS11841H07E50

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 11841H07E50

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Description & Specifications

Specifications

Protein Subtype Other Proteins
Gene Alias HCNTF, CNTF
Common Name Ciliary neurotrophic factor
Protein Tag His Tag
Molecular Weight 24.5kDa
Purity ≥90% by SDS-PAGE
Quantity 50μg
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Gene Symbol CNTF
Validated Application Assay Standard
Protein Family Cell Structure/Organelle Proteins
Expression System E. coli
For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized

Ciliary Neurotrophic Factor (CNTF), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

  • Activity: Measured by its binding ability in a functional ELISA: 1. Immobilized human CTNFR at 10μg/mL (100μL/well) can bind human CNTF with a linear ranger of 1.28-160ng/mL; 2. Immobilized Rat CTNFR at 10μg/mL (100μL/well) can bind human CNTF with a linear range of 1.28-160ng/mL
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.