Sino Biological™ CDH2 Recombinant Human Protein, His Tag
This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of human CDH2 (NP_001783.2) precursor (Met 1-Ala 724) fused to a polyhistidine tag at the C-terminus
Manufacturer: Life Technologies LS11039H08H25
Protein; Sino Biological; CDH2 Recombinant Human Protein, His Tag; 5 x 5ug; Source: Human; Purity: greater than or equal to 90% by SDS-PAGECadherin 2, type 1, N-cadherin (neuronal) (CDH2), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.
- Activity: Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells; when 5 x 104 cells/well are added to recombinant human Cadherin-2 coated plates (0.8μg/mL with 100μL/well), approximately >50% will adhere after 1 hour at 37°C
- Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
- Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible
This protein is manufactured by Sino Biological Inc.
|Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories|
|5 x 5μg|
|Intercellular Adhesion Molecule|
|CD325, CDHN, CDw325, NCAD, CDH2|
|Cell Structure/Organelle Proteins|
|≥90% by SDS-PAGE|
|Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.|
|CDH2 cadherin 2, type 1, N-cadherin (neuronal)|