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Sino Biological™ CD40LG Recombinant Human Protein, hIgG1-Fc Tag DFS Item


This recombinant protein was expressed from a DNA sequence encoding the soluble form of human CD40LG (NP_000065.1) (Met 113-Leu 261) fused to the Fc region of human IgG1 at the N-terminus

Manufacturer: life technologies  LS10239H01H50

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 10239H01H50

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Description & Specifications

Specifications

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Molecular Weight 42.8kDa
Purity ≥85% by SDS-PAGE
Quantity 50μg
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Protein Subtype TNF (Tumor Necrosis Factor)
Endotoxin Concentration <1 EU/μg
Gene Alias RP3-527F8.3, CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L
Common Name CD40 ligand
Gene Symbol CD40LG
Protein Tag IgG1 Fc Tag
Validated Application Assay Standard
Protein Family CD Proteins
Expression System Mammalian

CD40 ligand (CD40LG), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

  • Activity: Measured by its binding ability in a functional ELISA; immobilized human CD40 at 2μg/mL (100μL/well) can bind human CD40LG-Fc chimera with a linear range of 1.28-32ng/mL
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.