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Sino Biological™ CD40LG Recombinant Human Protein, hIgG1-Fc Tag DFS Item


This recombinant protein was expressed from a DNA sequence encoding the soluble form of human CD40LG (NP_000065.1) (Met 113-Leu 261) fused to the Fc region of human IgG1 at the N-terminus

Manufacturer: life technologies  LS10239H01H50

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 10239H01H50

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Description & Specifications

Specifications

Quantity 50μg
Molecular Weight 42.8kDa
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Purity ≥85% by SDS-PAGE
Common Name CD40 ligand
Format Lyophilized
Gene Symbol CD40LG
For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Expression System Mammalian
Protein Subtype TNF (Tumor Necrosis Factor)
Gene Alias RP3-527F8.3, CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L
Endotoxin Concentration <1 EU/μg
Protein Tag IgG1 Fc Tag
Protein Family CD Proteins
Validated Application Assay Standard

CD40 ligand (CD40LG), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

  • Activity: Measured by its binding ability in a functional ELISA; immobilized human CD40 at 2μg/mL (100μL/well) can bind human CD40LG-Fc chimera with a linear range of 1.28-32ng/mL
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.