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Sino Biological™ CD4 Recombinant Human Protein, His Tag DFS Item


This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Trp 390) of human CD4 (NP_000607.1) fused to a polyhistidine tag at the C-terminus

Manufacturer: life technologies  LS10400H08H25

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 10400H08H25

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Description & Specifications

Specifications

Purity ≥90% by SDS-PAGE
Format Lyophilized
Validated Application Assay Standard
Quantity 5 x 5μg
For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Structural Form Truncated/Recombinant/Cell Surface Protein
Common Name CD4 molecule
Endotoxin Concentration <1 EU/μg
Protein Tag His Tag
Protein Family CD Proteins
Storage Requirements Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Molecular Weight 42.2kDa
Gene ID (Entrez) 920
Gene Symbol CD4
Protein Subtype Glycoproteins
Expression System Mammalian
Gene Alias CD4mut

Cluster of Differentiation 4 (CD4), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.

  • Activity: 1. Measured by its ability to bind to HIV-1 gp120 in a functional ELISA; 2. Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells; when 5 x 104 cells/well are added to CD4 coated plates (2.5μg/mL and 100μL/well), approximately 60-80% will adhere specifically after 60 minutes at 37°C
  • Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.