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Sino Biological™ CD4 Recombinant Human Protein, His Tag DFS Item


This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Trp 390) of human CD4 (NP_000607.1) fused to a polyhistidine tag at the C-terminus

Manufacturer: life technologies  LS10400H08H25

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 10400H08H25

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Description & Specifications

Specifications

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Gene ID (Entrez) 920
Molecular Weight 42.2kDa
Purity ≥90% by SDS-PAGE
Quantity 5 x 5μg
Storage Requirements Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Structural Form Truncated/Recombinant/Cell Surface Protein
Protein Subtype Glycoproteins
Endotoxin Concentration <1 EU/μg
Gene Alias CD4mut
Common Name CD4 molecule
Gene Symbol CD4
Protein Tag His Tag
Validated Application Assay Standard
Protein Family CD Proteins
Expression System Mammalian

Cluster of Differentiation 4 (CD4), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.

  • Activity: 1. Measured by its ability to bind to HIV-1 gp120 in a functional ELISA; 2. Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells; when 5 x 104 cells/well are added to CD4 coated plates (2.5μg/mL and 100μL/well), approximately 60-80% will adhere specifically after 60 minutes at 37°C
  • Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.