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Sino Biological™ CD4 Recombinant Human Protein, hIgG1-Fc.His Tag DFS Item

This recombinant human protein is expressed from a DNA sequence encoding the extracellular domain (Met 1-Trp 390) of human CD4 (NP_000607.1) fused to the polyhistidine-tagged Fc region of human IgG1 at the C-terminus

Manufacturer: life technologies  LS10400H03H250

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For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 10400H03HL


Description & Specifications


For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Gene ID (Entrez) 920
Molecular Weight 68.7kDa
Purity ≥90% by SDS-PAGE
Quantity 5 x 50μg
Storage Requirements Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Structural Form Truncated/Recombinant/Cell Surface Protein
Protein Subtype Glycoproteins
Endotoxin Concentration <1 EU/μg
Gene Alias CD4mut
Common Name CD4 molecule
Gene Symbol CD4
Protein Tag His Tag/IgG1 Fc Tag
Validated Application Assay Standard
Protein Family CD Proteins
Expression System Mammalian

Cluster of Differentiation 4 (CD4) recombinant human protein is supplied as a lyophilized powder. This protein is suitable for use in the study of protein-protein interactions, and cell biology research applications. It can also be used as a protein standard.&line-break;Background: Human T-cell surface glycoprotein CD4, also known as T-cell surface antigen T4/Leu-3, is a single-pass type I membrane protein. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 amplifies the signal generated by the TCR. CD4 interacts directly with MHC class II molecules on the surface of the antigen presenting cell via its extracellular domain. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Therefore, medical professionals refer to the CD4 count to decide when to begin treatment for HIV-infected patients.

  • N-terminal Sequence Analysis: Lys26
  • Activity: measured by its binding ability in a functional ELISA; immobilized human CD4-His at 2μg/well has been shown to bind biotinylated HIV-1 GP120 with a linear range of 0.078-1.25μg/mL
  • Formulation: lyophilized in PBS, pH 7.4, 5% mannitol, 5% trehalose, and 0.02% Tween®-80
  • Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

Shipping Condition: Room Temperature. Manufactured by Sino Biological (China).