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Sino Biological™ CD3D & CD3E Recombinant Mouse Protein, hIgG1-Fc & hIgG1-Fc.His Tag DFS Item

CD3 Antigen delta and CD3 Antigen epsilon Heterodimer (CD3D & CD3E), recombinant mouse protein is supplied as a lyophilized powder

Manufacturer:  Life Technologies LSCT025M2303H25

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Catalog No. CT025M2303H


 

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Includes: One vial of lyophilized protein

Specifications

Description

Protein; Sino Biological; CD3D and CD3E Recombinant Mouse Protein, hIgG1-Fc and hIgG1-Fc.His tag; 5ug; Source: Mouse; Purity: greater than or equal to 85% by SDS-PAGE

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Ala 105) of mouse CD3D (P04235) fused to the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; a DNA sequence encoding the extracellular domain (Met 1-Asp 108) of mouse CD3E (P22646) fused to the C-terminal His-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse CD3D/CD3E heterodimer was purified.
  • Activity: This recombinant protein has not been tested
  • Formulation: 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.

Description & Specifications

Specifications

Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
12500/12501
PAGE
5μg
C-Terminal
<1 EU/μg
CD3 Antigen delta and CD3 Antigen epsilon Heterodimer
Assay Standard
Mammalian
Lyophilized
76kDa
≥85% by SDS-PAGE
Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Truncated/Recombinant
None
CD3D and CD3E
CD Proteins
His Tag/IgG1 Fc Tag/DYKDDDDK