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Sino Biological™ CD3D & CD3E Recombinant Human Protein, hIgG1-Fc & hIgG1-Fc.His Tag DFS Item


CD3D molecule delta and CD3E molecule epsilon (CD3-TCR complex) Heterodimer (CD3D & CD3E), recombinant human protein is supplied as a lyophilized powder

Manufacturer: life technologies  LSCT026H0323H5

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One vial of lyophilized protein

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. CT026H0323H

  • / Each
 

Description & Specifications

Specifications

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Gene ID (Entrez) 915/916
Molecular Weight 77.5kDa
Purification Method PAGE
Purity ≥90% by SDS-PAGE
Quantity 5μg
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Termination C-Terminal
Structural Form Truncated/Recombinant
Endotoxin Concentration <1 EU/μg
Gene Alias None
Common Name CD3D molecule delta and CD3E molecule epsilon (CD3-TCR complex) Heterodimer
Gene Symbol CD3D and CD3E
Protein Tag His Tag/IgG1 Fc Tag/DYKDDDDK
Validated Application Assay Standard
Protein Family CD Proteins
Expression System Mammalian

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Ala 105) of human CD3D (P04234) fused to the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; a DNA sequence encoding the extracellular domain (Met 1-Asp 126) of human CD3E (P07766) fused to the C-terminal His-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified.

  • Activity: This recombinant protein has not been tested
  • Formulation: 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.