Sino Biological™ CD3D & CD3E Recombinant Human Protein, hIgG1-Fc & hIgG1-Fc.His Tag
CD3D molecule delta and CD3E molecule epsilon (CD3-TCR complex) Heterodimer (CD3D & CD3E), recombinant human protein is supplied as a lyophilized powder
Manufacturer: Life Technologies LSCT026H0323H250
Protein; Sino Biological; CD3D and CD3E Recombinant Human Protein, hIgG1-Fc and hIgG1-Fc.His tag; 5 x 50ug; Source: Human; Purity: greater than or equal to 90% by SDS-PAGEThis recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Ala 105) of human CD3D (P04234) fused to the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; a DNA sequence encoding the extracellular domain (Met 1-Asp 126) of human CD3E (P07766) fused to the C-terminal His-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified.
- Activity: This recombinant protein has not been tested
- Formulation: 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
- Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible
This protein is manufactured by Sino Biological Inc.
|Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories|
|5 x 50μg|
|CD3D molecule delta and CD3E molecule epsilon (CD3-TCR complex) Heterodimer|
|≥90% by SDS-PAGE|
|Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.|
|CD3D and CD3E|
|His Tag/IgG1 Fc Tag/DYKDDDDK|