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Sino Biological™ CD34 Recombinant Human Protein, hIgG1-Fc Tag DFS Item


This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of the human CD34 precursor (NP_001020280.1) (Met 1-Thr 290) fused to the Fc region of human IgG1 at the C-terminus

Manufacturer: life technologies  LS10103H02H5

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 10103H02H5

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Description & Specifications

Specifications

Molecular Weight 54kDa
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Endotoxin Concentration <1 EU/μg
Gene Alias RP11-328D5.2, CD34
Gene Symbol CD34
Validated Application Assay Standard
Protein Subtype Glycoproteins
Protein Tag IgG1 Fc Tag
Expression System Mammalian
Purity ≥90% by SDS-PAGE
Quantity 5μg
Protein Family CD Proteins
For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Structural Form Truncated/Recombinant/Cell Surface Protein
Common Name CD34 molecule

Cluster of Differentiation 34 (CD34), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in performing cell based assays. It can also be used as a protein standard, or in other research applications.

  • Activity: Measured by the ability of the immobilized protein to support the adhesion of the HUVEC human umbilical vein endothelial cell line; when 4 x 104 cells/well are added to human CD34 coated plates (100μL/well of a 0.8μg/mL solution), >40 % will adhere after one hour at 37°C
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.