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Sino Biological™ CD34 Recombinant Human Protein, hIgG1-Fc Tag DFS Item

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of the human CD34 precursor (NP_001020280.1) (Met 1-Thr 290) fused to the Fc region of human IgG1 at the C-terminus

Manufacturer:  Life Technologies LS10103H02H5

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Catalog No. 10103H02H5


 

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Specifications

Description

Protein; Sino Biological; CD34 Recombinant Human Protein, hIgG1-Fc tag; 5ug; Source: Human; Purity: greater than or equal to 90% by SDS-PAGE

Cluster of Differentiation 34 (CD34), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in performing cell based assays. It can also be used as a protein standard, or in other research applications.
  • Activity: Measured by the ability of the immobilized protein to support the adhesion of the HUVEC human umbilical vein endothelial cell line; when 4 x 104 cells/well are added to human CD34 coated plates (100μL/well of a 0.8μg/mL solution), >40 % will adhere after one hour at 37°C
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.

Description & Specifications

Specifications

Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
54kDa
5μg
Truncated/Recombinant/Cell Surface Protein
<1 EU/μg
CD34 molecule
Assay Standard
Mammalian
Lyophilized
≥90% by SDS-PAGE
Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Glycoproteins
RP11-328D5.2, CD34
CD34
CD Proteins
IgG1 Fc Tag