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Sino Biological™ CD274 (PDL1) Recombinant Human Protein, hIgG1-Fc Tag DFS Item


This recombinant protein was expressed from a DNA sequence encoding the N-terminal segment (Met 1-Thr 239) of the extracellular domain of human CD274 (NP_054862.1) fused to the Fc region of human IgG1 at the C-terminus

Manufacturer: life technologies  LS10084H02H5

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 10084H02H5

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Description & Specifications

Specifications

Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Common Name CD274 molecule
Validated Application Assay Standard
Protein Family CD Proteins
Gene Symbol CD274 (PDL1)
Gene Alias B7-H, B7H1, mgC142294, mgC142296, PD-L1, PDCD1L1, PDCD1LG1, PDL1
Endotoxin Concentration <1 EU/μg
Molecular Weight 52kDa
Purity ≥90% by SDS-PAGE
Quantity 5μg
Protein Subtype Other Proteins
Protein Tag IgG1 Fc Tag
Expression System Mammalian

CD274 molecule (CD274), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

  • Activity: Measured by its binding ability in a functional ELISA; immobilized human PDCD1/PD1 at 10μg/mL (100μg/well) can bind recombinant human CD274/PDL1-Fc chimera with a linear range of 0.02-0.4μg/mL
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.