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Sino Biological™ CD274 (PDL1) Recombinant Human Protein, hIgG1-Fc Tag DFS Item


This recombinant protein was expressed from a DNA sequence encoding the N-terminal segment (Met 1-Thr 239) of the extracellular domain of human CD274 (NP_054862.1) fused to the Fc region of human IgG1 at the C-terminus

Manufacturer: life technologies  LS10084H02H25

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 10084H02H25

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Description & Specifications

Specifications

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Molecular Weight 52kDa
Purity ≥90% by SDS-PAGE
Quantity 5 x 5μg
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Protein Subtype Other Proteins
Endotoxin Concentration <1 EU/μg
Gene Alias B7-H, B7H1, mgC142294, mgC142296, PD-L1, PDCD1L1, PDCD1LG1, PDL1
Common Name CD274 molecule
Gene Symbol CD274 (PDL1)
Protein Tag IgG1 Fc Tag
Validated Application Assay Standard
Protein Family CD Proteins
Expression System Mammalian

CD274 molecule (CD274), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

  • Activity: Measured by its binding ability in a functional ELISA; immobilized human PDCD1/PD1 at 10μg/mL (100μg/well) can bind recombinant human CD274/PDL1-Fc chimera with a linear range of 0.02-0.4μg/mL
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.