Please login to your online account to display your discounted pricing

Sino Biological™ CD19 Recombinant Human Protein, His Tag DFS Item


This recombinant protein is expressed from a DNA sequence encoding the extracellular domain (Met 1-Lys 291) of human CD19 (AAB60697) fused to a polyhistidine tag at the C-terminus

Manufacturer: life technologies  LS11880H08H5

 View more versions of this product

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 11880H08H5

  • / Each
 

Description & Specifications

Specifications

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Gene ID (Entrez) 930
Molecular Weight 31.6kDa
Purity ≥90% by SDS-PAGE
Quantity 5μg
Storage Requirements Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Structural Form Truncated/Recombinant/Cell Surface Protein
Protein Subtype Other Proteins
Endotoxin Concentration <1 EU/μg
Gene Alias B4, mgC12802
Common Name CD19 molecule
Gene Symbol CD19
Protein Tag His Tag
Validated Application Assay Standard
Protein Family CD Proteins
Expression System Mammalian

Cluster of Differentiation 19 (CD19) recombinant human protein is supplied as a lyophilized powder. This protein is suitable for use in analysis of protein structure the study of protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.&line-break;Background: Cluster of Differentiation 19 (CD19), is a single-pass type I membrane protein which contains two Ig-like C2-type (immunoglobulinlike) domains. CD19 forms a complex with CD21, CD81 and CD225 in the membrane of mature B cells. It interacts with VAV. It interacts with GRB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378. CD19 also interacts with PLCG2 when phosphorylated on Tyr-409. CD19 is an important pan B cell marker and co-stimulatory protein in humans and mice. CD19 is a hallmark differentiation antigen of the B cell lineage and positively regulates antigen receptor signal transduction in mature B cells. CD19 defines signaling thresholds for the Ag receptor as well as other cell surface receptors that regulate B lymphocyte selection, activation, and differentiation. Defects in CD19 are a cause of hypogammaglobulinemia.

  • N-terminal Sequence Analysis: Pro 20
  • Activity: Measured by its binding ability in a functional ELISA; immobilized human CD19 at 2μg/mL (100μL/well) has been shown to bind biotinylated human CD9 with a linear range of 0.032-0.8μg/mL
  • Formulation: lyophilized in PBS, pH 7.4, 5% mannitol, 5% trehalose, and 0.02% Tween®-80
  • Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

Shipping Condition: Room Temperature. Manufactured by Sino Biological (China).