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Sino Biological™ ANPEP Recombinant Human Protein, His Tag DFS Item


This recombinant protein was expressed from a DNA sequence encoding the metalloprotease domain (Lys 69-Lys 967, 603 Met/Ile) of the mature form of human ANPEP (NP_001141.2) with the signal peptide at the N-terminus and a polyhistidine tag at the C-terminus

Manufacturer: life technologies  LS10051H08H250

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 10051H08HL

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Description & Specifications

Specifications

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Gene Alias ANPEP, APN, CD13, LAP1, PEPN, gp150
Protein Tag His Tag
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Purity ≥90% by SDS-PAGE
Endotoxin Concentration <1 EU/μg
Common Name Alanyl (membrane) aminopeptidase
Molecular Weight 104.2kDa
Quantity 5 x 50μg
Protein Subtype Proteases
Gene Symbol ANPEP
Validated Application Assay Standard
Protein Family CD Proteins
Expression System Mammalian
Format Lyophilized

Alanyl Amonipeptidase (ANPEP), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

  • Activity: Measured by its ability to cleave the fluorogenic peptide substrate, Ala-7-amido-4-methylcoumarin (Ala-AMC); the specific activity is >2500 pmoles/min./μg
  • Formulation: Lyophilized in 25mM MES, 0.15M NaCl, pH 6.5, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.