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Shrimp Alkaline Phosphatase (SAP)

Treat unincorporated dNTPs in PCR reactions to prepare templates for DNA sequencing or SNP analysis. Shrimp Alkaline Phosphatase (SAP) is useful in molecular biology applications such as the dephosphorylation of phosphorylated ends of DNA or RNA for subsequent use in cloning or end-labeling of probes.

$82.65 - $603.20

Specifications

For Use With (Application) Dephosphorylation of DNA prior to cloning
View More Specs
Products
Catalog Number Mfr. No. Quantity Price Quantity    

AF783901000

 
life technologies
LS783901000UN
1000 units Each for $150.80

AF78390500

 
life technologies
LS78390500UN
500 units Each for $82.65

AF783905000

 
life technologies
LS783905000UN
5000 units Each for $603.20
Description & Specifications

Specifications

For Use With (Application) Dephosphorylation of DNA prior to cloning

  • 100% heat-inactivated in 5 min at 65°C
  • Significantly improved storage stability at lower temperatures
  • Very high specific activity (see Fig. 3)
  • Removes 5’-phosphates from DNA, RNA, dNTPs, and proteins
  • Purified from a recombinant source
  • May be added directly to restriction enzyme digests
  • No vector purification necessary
  • Requires no supplemental zinc or other additives for activity
  • Works direct in many different buffers
  • Easy treatment of unincorporated dNTPs in PCR products prior to DNA sequencing or SNP analysis