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Thermo Scientific™ SacI (10 U/μL)
Cut at GAGCT^C sites with SacI restriction enzyme, which performs best at 37°C in its own unique buffer (Isoschizomers: Eco53kI, EcoICRI, Psp124BI, SstI).
Supplier: Thermo Scientific™ ER1135
Description
Lambda DNA digested with SacI, 0.7% agarose, 2 cleavage sites
conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Highlights
- Superior quality -stringent quality control and industry leading manufacturing process
- Convenient color-coded Five Buffer System
- Includes universal Tango buffer for double-digestions
- BSA premixed in reaction buffers
- Wide selection of restriction endonuclease specificities
Specifications
| 10 U/μL | |
| SacI | |
| Yes | |
| No | |
| Restriction Enzyme |
| Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive | |
| Unique Buffer (10X Buffer SacI) | |
| 37°C | |
| 2000 U | |
| Traditional Cloning |
For Research Use Only. Not for use in diagnostic procedures.