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ROCK2 Recombinant Human Protein DFS Item

ROCK2 Recombinant Human Protein

Manufacturer:  Life Technologies LSPV3759

Catalog No. PV3759



Description

Description

ROCK2, the predominant ROCK isoform in skeletal muscle, is an important regulator downstream of Rho GTPases.
Specifications

Specifications

NP_004841
Pharma and biopharma, protein assays and analysis, target and lead identification and validation
90.8kDa
90% by SDS-PAGE
–80°C
1 Each
ROCK2
ROCK2 was pre-diluted in enzyme dilution buffer and assayed in 50mM Tris (pH 7.5), 10mM MgCl2, 0.25mM EGTA, 0.1mM Na3VO4, 5mM β-glycerophosphate, 2.5mM DTT, 0.01% Triton™ X-100, 200μM ATP, 200μg/mL S6 peptide substrate (AKRRRLSSLRA) and trace [32P]-γ-ATP for 10 minutes at 30°C
Kinase and inhibitors
DMPK
ROCK2 Recombinant Human Protein
Human
0.38mg/mL
367, 9475
ROCK2 Recombinant Human Protein
10μg
For research use only. Not for use in diagnostic procedures
Serine/threonine kinase
ROCK2 Recombinant Human Protein
Kinase assay
Baculovirus
Amino acids 1-552
2180nmoles of phosphate transferred to S6 peptide substrate (AKRRRLSSLRA) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 1.67μg/mL
GST Tag
Documents

For Research Use Only. Not for use in diagnostic procedures.