Thermo Scientific™ RNase T1
RNase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues.
Manufacturer: Thermo Scientific™ EN0542
Thermo Scientific Rnase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues (see Figure 1 in Supporting Data). It cleaves the phosphodiester bond between the 3'-guanylic residue and the 5'-OH residue of adjacent nucleotides with the formation of corresponding intermediate 2', 3'-cyclic phosphate. The reaction products are 3'-GMP and oligonucleotides with a terminal 3'-GMP.
Rnase T1 does not require metal ions for activity.
- Removal of RNA from DNA preparations
- RNA sequencing
- Ribonuclease protection assays. Used in conjunction with RNase A
- Removal of RNA from recombinant protein preparations
- Determination of the level of RNA transcripts synthesized in vitro from DNA templates containing a "G-less cassette"
|E. coli cells with a cloned rntA gene of Aspergillus oryzae|
|metal ions Mg2+, Ca2+, Zn2+, Fe2+, Cu2+ (MgCl2 at 100 mM concentration is approx. 40% inhibitory, CaCl2 at 10 mM is approx. 30% inhibitory); mononucleotides (2 ft.-GMP, 3 ft.-GMP, etc.); guanilyl-2 ft.,5 ft.-guanosine is a specific inhibitor (4) Inactivat|
|11.2 kDa monomer|