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Thermo Scientific™ RNase T1


RNase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues.

Manufacturer: thermo scientific™  EN0542

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Catalog No. FEREN0542

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Description & Specifications

Specifications

Concentration 1,000U/µL
Inhibitors metal ions Mg2+, Ca2+, Zn2+, Fe2+, Cu2+ (MgCl2 at 100 mM concentration is approx. 40% inhibitory, CaCl2 at 10 mM is approx. 30% inhibitory); mononucleotides (2 ft.-GMP, 3 ft.-GMP, etc.); guanilyl-2 ft.,5 ft.-guanosine is a specific inhibitor (4) Inactivat
Source E. coli cells with a cloned rntA gene of Aspergillus oryzae
Molecular Weight 11.2 kDa monomer
Units 500,000U

Thermo Scientific Rnase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues (see Figure 1 in Supporting Data). It cleaves the phosphodiester bond between the 3'-guanylic residue and the 5'-OH residue of adjacent nucleotides with the formation of corresponding intermediate 2', 3'-cyclic phosphate. The reaction products are 3'-GMP and oligonucleotides with a terminal 3'-GMP.

Rnase T1 does not require metal ions for activity.

Applications

  • Removal of RNA from DNA preparations
  • RNA sequencing
  • Ribonuclease protection assays. Used in conjunction with RNase A
  • Removal of RNA from recombinant protein preparations
  • Determination of the level of RNA transcripts synthesized in vitro from DNA templates containing a "G-less cassette"