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Thermo Scientific™ RNase I


Ribonuclease I (RNase I) is an endoribonuclease that hydrolyzes single-stranded RNA to nucleoside 3'-monophosphates.

Manufacturer: thermo scientific™  EN0602

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Catalog No. FEREN0602

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Description & Specifications

Specifications

Concentration 10U/µL
Inhibitors SDS at 0.1% concentration irreversibly inactivates the enzyme
Source E.coli cells with a cloned rna gene of E.coli
Molecular Weight 29.6kDa monomer
Units 5,000U

Ribonuclease I (RNase I), an endoribonuclease, preferentially hydrolyzes single-stranded RNA to nucleoside 3'-monophosphates via nucleoside 2', 3'-cyclic monophosphate intermediates.

The enzyme does not require any metal ions for activity.

Highlights

  • Stable and active under a wide variety of reaction conditions
  • Can be heat inactivated in 30 minutes at 100°C