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Thermo Scientific™ RiboLock RNase Inhibitor


RiboLock RNase Inhibitor specifically inhibits the activity of RNases A, B, and C, protecting RNA under a variety of reaction conditions.

Manufacturer: thermo scientific™  EO0381

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Catalog No. FEREO0381

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Description & Specifications

Specifications

Concentration 40U/µL
Inhibitors common denaturants (SDS, urea and all oxidizing reagents (p-chloromercuribenzoate, dissolved oxygen, ions in their higher oxidation states) strongly inhibit RiboLock RNase Inhibitor and release the RNase bound
Source E.coli cells with a cloned gene encoding mammalian ribonuclease inhibitor
Molecular Weight 49.6kDa monomer
Units 2,500U

RiboLock RNase Inhibitor inhibits the activity of RNases A , B, and C by binding them in a noncompetitive mode at a 1:1 ratio. It does not inhibit eukaryotic RNases T1, T2, U1, U2, CL3 as well as prokaryotic RNases I and H.

  • Does not inhibit eukaryotic RNases T1, T2, U1, U2, CL3 as well as prokaryotic RNases I and H
  • Performs under a wide range of reaction conditions
  • Protects RNA from degradation at temperatures up to 55°C
Quality Control:
  • The absence of ribonucleases, endo-, exodeoxyribonucleases and phosphatases and ribonucleases confirmed by appropriate quality tests
  • Functionally tested in RNA and cDNA synthesis
Source:
  • E.coli cells with a cloned gene encoding mammalian ribonuclease inhibitor
Molecular Weight:
  • 49.6kDa monomer Definition of Activity Unit:
    • One unit of the RiboLock RNase Inhibitor inhibits the activity of 5ng RNase A by 50%
    • Inhibitor activity is assayed in the following mixture: 100mM Tris-HCl (pH 7.5), 1.2mM EDTA, 0.1mg/mL BSA, 100ng/mL RNase A, 0.1mg/mL [3H]-RNA, 50mg/mL yeast RNA, 8mM DTT
    Storage Buffer:
    • The enzyme is supplied in: 20mM HEPES-NaOH (pH 7.5), 50mM NaCl, 8mM DTT, 0.5mM ELUGENT Detergent and 50% (v/v) glycerol
    Inhibition and Inactivation
    • Inhibitors: common denaturants (SDS, urea and all oxidizing reagents (p-chloromercuribenzoate, dissolved oxygen, ions in their higher oxidation states) strongly inhibit RiboLock RNase Inhibitor and release the RNase bound
    • Inactivated by heating at 75°C for 10 min. Residual activity detectable after 10 min. heating at 70°

    Recommended for:

    • Inhibition of RNA degradation in the following: in vitrotranscription (1), cDNA synthesis (2), in vitrotranslation (3), isolation of mammalian cell fractions that contain mRNA-protein complex
    • RNA purification and storage
    • Separation and identification of specific ribonuclease activities
    • Studies of tumor suppression

    Note:

    • DTT provided in the Storage Buffer ensures stability during long term storage, but is not necessary for inhibitor activity
    • Recommended concentration 1u/μl of a reaction mixture