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Thermo Scientific™ RevertAid H Minus Reverse Transcriptase


A recombinant M-MuLV RT lacking RNase H activity for high yield cDNA synthesis.

Manufacturer: thermo scientific™  EP0452

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Catalog No. FEREP0452

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Description & Specifications

Specifications

For Use With (Application) First strand cDNA synthesis for RT-PCR and real-time RT-PCR (3, 4, 5); Reverse transcription at elevated temperatures to reduce effects of secondary structure; Synthesis of cDNA for cloning and expression
Includes 5 x 1mL 5X Reaction Buffer
Product Name RevertAid H Minus Reverse Transcriptase
Quantity 100U

RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain. RevertAid H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA, and therefore high yields of full-length cDNA from long templates are obtained. RevertAid H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 50°C).

Highlights

  • High yields of full-length first strand cDNA up to 13 kb
  • Optimum activity at 42 to 45°C
  • Active up to 55°C
  • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)