Thermo Scientific™ RevertAid H Minus Reverse Transcriptase
A recombinant M-MuLV RT lacking RNase H activity for high yield cDNA synthesis.
Manufacturer: Thermo Scientific™ EP0452
RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain. RevertAid H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA, and therefore high yields of full-length cDNA from long templates are obtained. RevertAid H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 50°C).
- High yields of full-length first strand cDNA up to 13 kb
- Optimum activity at 42 to 45°C
- Active up to 55°C
- Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)
|First strand cDNA synthesis for RT-PCR and real-time RT-PCR (3, 4, 5); Reverse transcription at elevated temperatures to reduce effects of secondary structure; Synthesis of cDNA for cloning and expression|
|5 x 1mL 5X Reaction Buffer|
|RevertAid H Minus Reverse Transcriptase|