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Thermo Scientific™ RevertAid H Minus First Strand cDNA Synthesis Kit


Perform efficient synthesis of first strand cDNA from RNA templates with this complete synthesis system.

Manufacturer: thermo scientific™  K1631

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Catalog No. FERK1631

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Description & Specifications

Specifications

For Use With (Application) First strand cDNA synthesis for RT-PCR and real-time RT-PCR (1, 2); Construction of full length cDNA libraries; Generation of probes for hybridization; aRNA synthesis.
Product Type RevertAid™ H Minus First Strand cDNA Synthesis Kit
Sufficient For 20 reactions

RevertAid H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit includes RevertAid H Minus Reverse Transcriptase, which has a point mutation that completely eliminates RNase H activity. Therefore, it does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA.

Highlights

  • High yields of full-length first strand cDNA up to 13 kb
  • Increased reaction temperatures in the range of 42 to 55°C
  • Supplied with the recombinant RiboLock RNase Inhibitor
  • Complete kit - oligo(dT)18 and random hexamer primers included with the kit