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Thermo Scientific™ Restore™ Western Blot Stripping Buffer


Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.

Manufacturer: thermo scientific™  21059

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Catalog No. PI21059

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Description & Specifications

Specifications

Description Restore Western Blot Stripping Buffer
Formulation Proprietary clear, colorless, acidic solution
Kit Contents Single component
Quantity 500mL
Sufficient For 25 mini blots or 2000cm2 membrane
Storage Requirements Room Temperature or 4°C

Thermo Scientific™ Restore Western Blot Stripping Buffer safely and effectively removes primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.

Performing gel electrophoresis and duplicate immunoblot assays to test new primary antibodies or antibody concentrations is time-consuming and expensive. Restore Western Blot Stripping Buffer eliminates this waste when detecting immunoblots with chemiluminescent Western blotting substrates. The reagent allows clean and efficient removal of primary and secondary antibodies from immunoblots without removing or damaging the immobilized antigen. This allows blots to be stripped and reprobed with confidence.

Highlights:

  • Saves time – no need to re-run gels and blots

  • Saves costly sample – re-probe the membrane using the same target sample

  • Effective – formulation is more efficient at stripping antibodies than homemade buffers

  • Gentle – does not damage the target antigen during stripping allowing efficient reprobing

  • Odor-free – no mercaptans means no acrid odors

  • Economical – less expensive than other commercial stripping buffers

Reuse a nitrocellulose or PVDF blot to detect a different target with a different primary antibody; Reprobe a blot to correct or optimize antibody concentrations that were ineffective the first time.