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Thermo Scientific™ Pierce™ Rat TNF alpha ELISA Kits

Measure levels of rat tumor necrosis factor alpha (TNFa) levels in rat serum, plasma and culture supernatant samples using 96-well plates.

$651.05 - $1,145.50

Specifications

Assay Range 31 to 2500pg/mL
Description Rat TNF alpha ELISA Kit
Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement Absorbance at 450nm minus absorbance at 550nm
Precision Intra-assay CV <10%; inter-assay CV <10%
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Products ${productFamilyLength}
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
ENER3TNFA Thermo Scientific™ Pierce™
ER3TNFA
1 plate kit Each for $651.05
ENER3TNFA2 Thermo Scientific™ Pierce™
ER3TNFA2
2 plate kit Each for $1,145.50
Specifications

Description

Assay TNFα (rat tumor necrosis factor alpha) levels in rat serum, plasma (EDTA, heparin) and culture supernatant samples with our Thermo Scientific Rat TNF alpha ELISA Kits.

These rat TNF alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant rat TNFα (rat tumor necrosis factor alpha)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 31 to 2500pg/mL

  • Sensitivity: < 15pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human TNFα, human TNFβ or porcine TNFα. Mouse TNFα cross-reacts weakly (< 3%)

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Description & Specifications

Specifications

31 to 2500pg/mL
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Intra-assay CV <10%; inter-assay CV <10%
Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
No significant cross-reaction with human TNFα, human TNFβ or porcine TNFα. Mouse TNFα cross-reacts weakly (< 3%)
3 hr.
Rat TNF alpha ELISA Kit
Absorbance at 450nm minus absorbance at 550nm
50µL
<15pg/mL
Natural and recombinant rat TNFa (rat tumor necrosis factor alpha)