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Thermo Scientific™ Pierce™ Rat MCP-1 ELISA Kits

Measure levels of rat monocyte chemoattractant protein-1 (MCP1) in rat serum, plasma and cell culture supernatant samples.

Manufacturer:  Thermo Scientific™ Pierce™ PIERMCP1

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Catalog No. ENERMCP1


 

Specifications

Description

Assay MCP-1 (rat monocyte chemoattractant protein-1) levels in rat serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Rat MCP-1 ELISA Kits.

These rat MCP-1 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of monocyte chemoattractant protein-1 (MCP-1) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant rat MCP-1 (monocyte chemoattractant protein-1)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 38 to 1500pg/mL

  • Sensitivity: < 5pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with rat IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-10, MIP-1α, RANTES, mouse MCP-1, or human MCP-1

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Description & Specifications

Specifications

<5pg/mL
30X Wash Buffer, 50mL
Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Complete ELISA kit with coated 96-well plate
No significant cross-reaction with rat IFNg, IL-1a, IL-1ß, IL-2, IL-4, IL-6, IL-10, MIP-1a, RANTES, mouse MCP-1, or human MCP-1
50.0µL
96 microplate wells
1 plate kit
Intra-assay CV <10%; inter-assay CV <10%
38 to 1500pg/mL
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Absorbance at 450nm minus absorbance at 550nm
3 hr.
Natural and recombinant rat MCP-1 (monocyte chemoattractant protein-1)
Rat MCP-1 ELISA Kit