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Thermo Scientific™ Pierce™ Rat IL-10 ELISA Kits


Measure levels of rat interleukin-10 in rat serum, plasma (EDTA, heparin, sodium citrate) and cell culture supernatant samples using 96-well plates.

Manufacturer: thermo scientific™ pierce™  ERIL10

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Catalog No. ENERIL10

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Description & Specifications

Specifications

Assay Range 16 to 500pg/mL
Description Rat IL-10 ELISA Kit
Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Formulation Complete ELISA kit with coated 96-well plate
Kit Contents 30X Wash Buffer, 50mL
Precision Dependent upon sample type (generally less than 10% CV)
Measurement Absorbance at 450nm minus absorbance at 550nm
Quantity 1 plate kit
Sensitivity <3pg/mL
Sample Type Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Sample Size 50µL
Specificity No significant cross-reaction with rat IFNg, IL-1a, IL-1ß, IL-2, IL-4, IL-6, MCP-1, MIP-1a, RANTES, TNFa, human IL-10, or mouse IL-10
Sufficient For 96 microplate wells
Total Assay Time 3 hr.
Target Natural and recombinant rat interleukin-10 (IL-10)

Assay IL-10 (rat interleukin-10) levels in rat serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific™ Rat IL-10 ELISA Kits.

These rat IL-10 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-10 (IL-10) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution.The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

  • Target: Natural and recombinant rat interleukin-10 (IL-10)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 16 to 500pg/mL

  • Sensitivity: < 3pg/mL

  • Precision: Dependent upon sample type (generally less than 10% CV)

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with rat IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-6, MCP-1, MIP-1α, RANTES, TNFα, human IL-10, or mouse IL-10

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.