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Thermo Scientific™ Pierce™ Rat IL-1 beta ELISA Kits

Measure levels of rat interleukin-1 beta (IL-1b) in rat serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples using 96-well plates.

Manufacturer:  Thermo Scientific™ Pierce™ PIER2IL1B

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Catalog No. ENER2IL1B


 

Specifications

Description

Assay IL-1β (rat interleukin-1 beta) levels in rat serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Rat IL-1 beta ELISA Kits.

These rat IL-1 beta ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-1 beta (IL-1β) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant rat interleukin-1 beta (IL-1β)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 25.6 to 2500pg/mL

  • Sensitivity: < 12pg/mL

  • Precision: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3.5 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with rat IFNγ, IL-1α, IL-2, IL-4, IL-6, IL-10, MCP-1, MIP-1α, or RANTES, or human, mouse or pig IL-1β

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Description & Specifications

Specifications

<12pg/mL
Complete ELISA kit with coated 96-well plate
96 microplate wells
Rat IL-1 beta ELISA Kit
50µL
Natural and recombinant rat interleukin-1 beta (IL-1ß)
Absorbance at 450nm minus absorbance at 550nm
1 plate kit
Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
No significant cross-reaction with rat IFNg, IL-1a, IL-2, IL-4, IL-6, IL-10, MCP-1, MIP-1a, or RANTES, or human, mouse or pig IL-1ß
30X Wash Buffer, 50mL
Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
25.6 to 2500pg/mL
3.5 hr.
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate